CATH Classification

Domain Context

CATH Clusters

Superfamily Cytochrome P450
Functional Family Cytochrome P450 monooxygenase PikC

Enzyme Information

1.14.15.33
Pikromycin synthase.
based on mapping to UniProt O87605
(1) Narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = pikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O. (2) Narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = neopikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O. (3) Narbomycin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H(+) + 2 O(2) = novapikromyin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O. (4) 10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O. (5) 10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = neomethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O. (6) 10-deoxymethymycin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H(+) + 2 O(2) = novamethymycin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O.
-!- A cytochrome P450 (heme-thiolate) protein. -!- Involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit. -!- With narbomycin it hydroxylates at either C-12 to give pikromycin or C-14 to give neopikromycin or both positions to give narvopikromycin. -!- With 10-deoxymethymycin it hydroxylates at either C-10 to give methymycin or C-12 to give neomethymycin or both positions to give novamethymycin. -!- Formerly EC 1.14.13.185.

UniProtKB Entries (1)

O87605
PIKC_STRVZ
Streptomyces venezuelae
Cytochrome P450 monooxygenase PikC

PDB Structure

PDB 4B7D
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Recognition of Synthetic Substrates by P450 Pikc
Podust, L.M.
To be Published