CATH Classification

Domain Context

CATH Clusters

Superfamily Tetratricopeptide repeat domain
Functional Family UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit

Enzyme Information

2.4.1.255
Protein O-GlcNAc transferase.
based on mapping to UniProt O15294
(1) UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine. (2) UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine.
-!- Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. -!- EC 2.4.1.255 transfers GlcNAc onto substrate proteins and EC 3.2.1.169 cleaves GlcNAc from the modified proteins.

UniProtKB Entries (1)

Q15750
TAB1_HUMAN
Homo sapiens
TGF-beta-activated kinase 1 and MAP3K7-binding protein 1

PDB Structure

PDB 4AY6
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
O-Glcnac Transferase Invokes Nucleotide Sugar Pyrophosphate Participation in Catalysis
Schimpl, M., Zheng, X., Borodkin, V.S., Blair, D.E., Ferenbach, A.T., Schuettelkopf, A.W., Navratilova, I., Aristotelous, T., Albarbarawi, O., Robinson, D.A., Macnaughtan, M.A., Van Aalten, D.M.F.
Nat.Chem.Biol.