CATH Classification

Domain Context

CATH Clusters

Superfamily Diphtheria toxin, translocation domain
Functional Family Diphtheria toxin

Enzyme Information

2.4.2.36
NAD(+)--diphthamide ADP-ribosyltransferase.
based on mapping to UniProt P00588
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2].
-!- Dihtheria toxin and some other bacterial toxins catalyze this reaction, which inactivates translation elongation factor 2 (EF2). -!- The acceptor is diphthamide, a unique modification of a histidine residue in the elongation factor found in archaebacteria and all eukaryotes, but not in eubacteria. -!- Cf. EC 2.4.2.31. -!- The relevant histidine of EF2 is His(715) in mammals, His(699) in yeast and His(600) in Pyrococcus horikoshii.

UniProtKB Entries (1)

P00588
DTX_CORBE
Corynephage beta
Diphtheria toxin

PDB Structure

PDB 4AE1
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural Basis for Lack of Toxicity of the Diphtheria Toxin Mutant Crm197.
Malito, E., Bursulaya, B., Chen, C., Surdo, P.L., Picchianti, M., Balducci, E., Biancucci, M., Brock, A., Berti, F., Bottomley, M.J., Nissum, M., Costantino, P., Rappuoli, R., Spraggon, G.
Proc.Natl.Acad.Sci.USA