CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.50 | Rossmann fold |
|
3.40.50.720 | NAD(P)-binding Rossmann-like Domain |
Domain Context
CATH Clusters
| Superfamily | NAD(P)-binding Rossmann-like Domain |
| Functional Family |
Enzyme Information
| 1.1.1.304 |
Diacetyl reductase ((S)-acetoin forming).
based on mapping to UniProt Q48436
(S)-acetoin + NAD(+) = diacetyl + NADH.
-!- The reaction is catalyzed in the reverse direction. -!- This activity is usually associated with butanediol dehydrogenase activity (EC 1.1.1.4 or EC 1.1.1.76). -!- While the butanediol dehydrogenase activity is reversible, diacetyl reductase activity is irreversible. -!- This enzyme has been reported in the bacteria Geobacillus stearothermophilus, Enterobacter aerogenes and Klebsiella pneumoniae. -!- Different from EC 1.1.1.303. -!- Formerly EC 1.1.1.5.
|
| 1.1.1.76 |
(S,S)-butanediol dehydrogenase.
based on mapping to UniProt Q9ZNN8
(2S,3S)-butane-2,3-diol + NAD(+) = (S)-acetoin + NADH.
-!- This enzyme catalyzes the reversible reduction of (S)-acetoin to (S,S)-butane-2,3-diol. -!- It can also catalyze the irreversible reduction of diacetyl to (S)- acetoin.
|
| 1.1.1.304 |
Diacetyl reductase ((S)-acetoin forming).
based on mapping to UniProt Q9ZNN8
(S)-acetoin + NAD(+) = diacetyl + NADH.
-!- The reaction is catalyzed in the reverse direction. -!- This activity is usually associated with butanediol dehydrogenase activity (EC 1.1.1.4 or EC 1.1.1.76). -!- While the butanediol dehydrogenase activity is reversible, diacetyl reductase activity is irreversible. -!- This enzyme has been reported in the bacteria Geobacillus stearothermophilus, Enterobacter aerogenes and Klebsiella pneumoniae. -!- Different from EC 1.1.1.303. -!- Formerly EC 1.1.1.5.
|
UniProtKB Entries (2)
| Q9ZNN8 |
BUDC_CORGT
Corynebacterium glutamicum
L-2,3-butanediol dehydrogenase
|
| Q48436 |
BUDC_KLEPN
Klebsiella pneumoniae
Diacetyl reductase [(S)-acetoin forming]
|
PDB Structure
| PDB | 3WYE |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Crystal Structure of chimeric engineered (2S,3S)-butanediol dehydrogenase complexed with NAD+
To be Published
|