CATH Classification

Domain Context

CATH Clusters

Superfamily 2.60.40.3390
Functional Family

Enzyme Information

2.4.99.21
Dolichyl-phosphooligosaccharide-protein glycotransferase.
based on mapping to UniProt O29867
An archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
-!- The enzyme, characterized from the archaea Methanococcus voltae and Haloferax volcanii, transfers a glycan component from dolichyl phosphooligosaccharide to external proteins. -!- It is different from EC 2.4.99.18 which uses dolichyl diphosphate as carrier compound in bacteria and eukaryotes. -!- The enzyme participates in the N-glycosylation of proteins in some archaea. -!- Dolichol used by archaea is different from that used by eukaryotes; it is much shorter (C(55)-C(60)), it is alpha,omega-saturated and it may have additional unsaturated positions in the chain.

UniProtKB Entries (1)

O29867
AGLB3_ARCFU
Archaeoglobus fulgidus DSM 4304
Dolichyl-phosphooligosaccharide-protein glycotransferase 3

PDB Structure

PDB 3WAK
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation
Matsumoto, S., Shimada, A., Nyirenda, J., Igura, M., Kawano, Y., Kohda, D.
Proc.Natl.Acad.Sci.USA