CATH Classification

Domain Context

CATH Clusters

Superfamily 3.90.1200.10
Functional Family

Enzyme Information

2.7.1.163
Hygromycin B 4-O-kinase.
based on mapping to UniProt P00557
ATP + hygromycin B = ADP + 4-O-phosphohygromycin B.
-!- Phosphorylates the antibiotic hygromycin B. -!- Whereas the enzyme from Streptomyces hygroscopicus (EC 2.7.1.119) catalyzes the formation of 7''-O-phosphohygromycin B, this enzyme, found in Escherichia coli carrying a plasmid conferring resistance to hygromycin-B, forms 4-O-phosphohygromycin B. -!- Formerly EC 2.7.1.n6.

UniProtKB Entries (1)

P00557
KHYB_ECOLX
Escherichia coli
Hygromycin-B 4-O-kinase

PDB Structure

PDB 3W0N
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal structures of the ternary complex of APH(4)-Ia/Hph with hygromycin B and an ATP analog using a thermostable mutant.
Iino, D., Takakura, Y., Fukano, K., Sasaki, Y., Hoshino, T., Ohsawa, K., Nakamura, A., Yajima, S.
J.Struct.Biol.