CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.420.40
Functional Family nebramycin 5' synthase

Enzyme Information

6.1.2.2
Nebramycin 5' synthase.
based on mapping to UniProt Q70IY1
(1) Tobramycin + carbamoyl phosphate + ATP + H(2)O = nebramycin 5' + AMP + diphosphate + phosphate. (2) Kanamycin A + carbamoyl phosphate + ATP + H(2)O = 6''-O- carbamoylkanamycin A + AMP + diphosphate + phosphate.
-!- The enzyme from the bacterium Streptoalloteichus tenebrarius catalyzes the activation of carbamoyl phosphate to O-carbamoyladenylate and the subsequent carbamoylation of kanamycin and tobramycin. -!- Formerly EC 2.1.3.13 and EC 2.1.3.14.

UniProtKB Entries (1)

Q70IY1
TOBZ_STRSD
Streptoalloteichus tenebrarius
Nebramycin 5' synthase

PDB Structure

PDB 3VES
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The O-Carbamoyltransferase TobZ Catalyzes an Ancient Enzymatic Reaction.
Parthier, C., Gorlich, S., Jaenecke, F., Breithaupt, C., Brauer, U., Fandrich, U., Clausnitzer, D., Wehmeier, U.F., Bottcher, C., Scheel, D., Stubbs, M.T.
Angew.Chem.Int.Ed.Engl.