CATH Classification

Domain Context

CATH Clusters

Superfamily main proteinase (3clpro) structure, domain 3
Functional Family Replicase polyprotein 1a

Enzyme Information

Cysteine endopeptidases.
based on mapping to UniProt P0C6U8
SARS coronavirus main proteinase.
based on mapping to UniProt P0C6U8
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
-!- SARS coronavirus main protease is the key enzyme in SARS coronavirus replicase polyprotein processing. -!- Belongs to peptidase family C30.
Ubiquitinyl hydrolase 1.
based on mapping to UniProt P0C6U8
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (1)

SARS coronavirus
Replicase polyprotein 1a

PDB Structure

External Links
Primary Citation
Design, synthesis and crystallographic analysis of nitrile-based broad-spectrum peptidomimetic inhibitors for coronavirus 3C-like proteases
Chuck, C.P., Chen, C., Ke, Z., Wan, D.C.-C., Chow, H.F., Wong, K.B.