CATH Classification

Domain Context

CATH Clusters

Superfamily Vaccinia Virus protein VP39
Functional Family Ribosomal RNA large subunit methyltransferase K/L

Enzyme Information

2.1.1.173
23S rRNA (guanine(2445)-N(2))-methyltransferase.
based on mapping to UniProt P75864
S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L- homocysteine + N(2)-methylguanine(2445) in 23S rRNA.
-!- The enzyme methylates 23S rRNA in vitro, assembled 50S subunits are not a substrate. -!- The enzyme specifically methylates guanine(2445) at N(2) in 23S rRNA. -!- Formerly EC 2.1.1.52.
2.1.1.264
23S rRNA (guanine(2069)-N(7))-methyltransferase.
based on mapping to UniProt P75864
S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L- homocysteine + N(7)-methylguanine(2069) in 23S rRNA.
-!- The enzyme specifically methylates guanine(2069) at position N7 in 23S rRNA. -!- In gamma-proteobacteria the enzyme also catalyzes EC 2.1.1.173, while in beta-proteobacteria the activities are carried out by separate proteins. -!- The enzyme from the gamma-proteobacterium Escherichia coli has RNA unwinding activity as well.

UniProtKB Entries (1)

P75864
RLMKL_ECOLI
Escherichia coli K-12
Ribosomal RNA large subunit methyltransferase K/L

PDB Structure

PDB 3V97
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA
Wang, K.T., Desmolaize, B., Nan, J., Zhang, X.W., Li, L.F., Douthwaite, S., Su, X.D.
Nucleic Acids Res.