CATH Classification

Domain Context

CATH Clusters

Superfamily Nitric Oxide Synthase;Heme Domain;Chain A domain 2
Functional Family Nitric oxide synthase, endothelial

Enzyme Information

1.14.13.39
Nitric-oxide synthase (NADPH).
based on mapping to UniProt P29476
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
-!- This eukaryotic enzyme, which is found in plants and animals, consists of oxygenase and reductase domains that are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- May produce superoxide under certain conditions. -!- Cf. EC 1.14.13.165.

UniProtKB Entries (1)

P29476
NOS1_RAT
Rattus norvegicus
Nitric oxide synthase, brain

PDB Structure

PDB 3UFP
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Selective monocationic inhibitors of neuronal nitric oxide synthase. Binding mode insights from molecular dynamics simulations.
Huang, H., Ji, H., Li, H., Jing, Q., Labby, K.J., Martasek, P., Roman, L.J., Poulos, T.L., Silverman, R.B.
J.Am.Chem.Soc.