CATH Classification

Domain Context

CATH Clusters

Superfamily Ribosomal RNA adenine dimethylase-like, domain 2
Functional Family Ribosomal RNA small subunit methyltransferase A

Enzyme Information

2.1.1.182
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase.
based on mapping to UniProt P06992
4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)- dimethyladenine(1519) in 16S rRNA.
-!- KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of adenine(1518) and adenine(1519) in 16S rRNA. -!- Strains lacking the methylase are resistant to kasugamycin. -!- Formerly EC 2.1.1.48.

UniProtKB Entries (1)

P06992
RSMA_ECOLI
Escherichia coli K-12
Ribosomal RNA small subunit methyltransferase A

PDB Structure

PDB 3TPZ
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Control of Substrate Specificity by a Single Active Site Residue of the KsgA Methyltransferase.
O'Farrell, H.C., Musayev, F.N., Scarsdale, J.N., Rife, J.P.
Biochemistry