CATH Classification

Domain Context

CATH Clusters

Superfamily Alpha-ketoglutarate-dependent dioxygenase AlkB-like
Functional Family Alpha-ketoglutarate-dependent dioxygenase AlkB

Enzyme Information

1.14.11.33
DNA oxidative demethylase.
based on mapping to UniProt P05050
DNA-base-CH(3) + 2-oxoglutarate + O(2) = DNA-base + formaldehyde + succinate + CO(2).
-!- Activity is slightly stimulated by ascorbate. -!- Catalyzes oxidative demethylation of the DNA base lesions N(1)- methyladenine, N(3)-methylcytosine, N(1)-methylguanine, and N(3)- methylthymine. -!- It works better on single-stranded DNA (ssDNA) and is capable of repairing damaged bases in RNA.

UniProtKB Entries (1)

P05050
ALKB_ECOLI
Escherichia coli K-12
Alpha-ketoglutarate-dependent dioxygenase AlkB

PDB Structure

PDB 3T3Y
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Dynamic combinatorial mass spectrometry leads to inhibitors of a 2-oxoglutarate-dependent nucleic Acid demethylase.
Woon, E.C., Demetriades, M., Bagg, E.A., Aik, W., Krylova, S.M., Ma, J.H., Chan, M., Walport, L.J., Wegman, D.W., Dack, K.N., McDonough, M.A., Krylov, S.N., Schofield, C.J.
J.Med.Chem.