CATH Classification

Domain Context

CATH Clusters

Superfamily effector protein (NleL)
Functional Family SPI-1 type III secretion system effector HECT-type E3 ubiquitin transferase SopA

Enzyme Information

2.3.2.26
HECT-type E3 ubiquitin transferase.
based on mapping to UniProt Q8ZNR3
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (2)

P68036
UB2L3_HUMAN
Homo sapiens
Ubiquitin-conjugating enzyme E2 L3
Q8ZNR3
SOPA_SALTY
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
E3 ubiquitin-protein ligase SopA

PDB Structure

PDB 3SY2
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal structures of two bacterial HECT-like E3 ligases in complex with a human E2 reveal atomic details of pathogen-host interactions.
Lin, D.Y., Diao, J., Chen, J.
Proc.Natl.Acad.Sci.USA