CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.1190.20
Functional Family ADP-dependent (S)-NAD(P)H-hydrate dehydratase

Enzyme Information

4.2.1.136
ADP-dependent NAD(P)H-hydrate dehydratase.
based on mapping to UniProt P94368
(1) ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine- dinucleotide = AMP + phosphate + NADH. (2) ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine- dinucleotide phosphate = AMP + phosphate + NADPH.
-!- Acts equally well on hydrated NADH and hydrated NADPH. -!- NAD(P)H spontaneously hydrates to both the (6S)- and (6R)- isomers. -!- The enzyme from bacteria consists of two domains, one of which acts as an NAD(P)H-hydrate epimerase that interconverts the two isomers to a 60:40 ratio (cf. EC 5.1.99.6), while the other catalyzes the dehydration. -!- Hence the enzyme can restore the complete mixture of isomers into NAD(P)H. -!- The enzyme has no activity with ATP, contrary to the enzyme from eukaryotes (cf. EC 4.2.1.93).

UniProtKB Entries (1)

P94368
NNRD_BACSU
Bacillus subtilis subsp. subtilis str. 168
ADP-dependent (S)-NAD(P)H-hydrate dehydratase

PDB Structure

PDB 3RQX
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Identification of unknown protein function using metabolite cocktail screening.
Shumilin, I.A., Cymborowski, M., Chertihin, O., Jha, K.N., Herr, J.C., Lesley, S.A., Joachimiak, A., Minor, W.
Structure