CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.11890
Functional Family (R)-2-hydroxyisocaproyl-CoA dehydratase beta subunit

Enzyme Information

4.2.1.157
(R)-2-hydroxyisocaproyl-CoA dehydratase.
based on mapping to UniProt Q5U923
(R)-2-hydroxy-4-methylpentanoyl-CoA = 4-methylpent-2-enoyl-CoA + H(2)O.
-!- The enzyme, isolated from the bacterium Peptoclostridium difficile, is involved in the reductive branch of L-leucine fermentation. -!- It catalyzes an alpha/beta-dehydration, which depends on the reductive formation of ketyl radicals on the substrate generated by injection of a single electron from the ATP-dependent activator protein HadI.

UniProtKB Entries (1)

Q5U923
HADC_CLODI
Clostridioides difficile
(R)-2-hydroxyisocaproyl-CoA dehydratase beta subunit

PDB Structure

PDB 3O3O
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural Basis for Reductive Radical Formation and Electron Recycling in (R)-2-Hydroxyisocaproyl-CoA Dehydratase.
Knauer, S.H., Buckel, W., Dobbek, H.
J.Am.Chem.Soc.