CATH Classification

Domain Context

CATH Clusters

Superfamily Zn peptidases
Functional Family Allantoate amidohydrolase

Enzyme Information
N-carbamoyl-L-amino-acid hydrolase.
based on mapping to UniProt Q53389
N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H(2)O = L-2-amino acid + NH(3) + CO(2).
-!- This enzyme, along with EC, EC and EC, forms part of the reaction cascade known as the 'hydantoinase process', which allows the total conversion of D,L-5-monosubstituted hydantoins into optically pure D- or L-amino acids. -!- The enzyme from Alcaligenes xylosoxidans has broad specificity for carbamoyl-L-amino acids, although it is inactive on the carbamoyl derivatives of glutamate, aspartate, arginine, tyrosine or tryptophan. -!- The enzyme from Sinorhizobium meliloti requires a divalent cation for activity and can hydrolyze N-carbamoyl-L-tryptophan as well as N-carbamoyl L-amino acids with aliphatic substituents. -!- The enzyme is inactive on derivatives of D-amino acids. -!- In addition to N-carbamoyl L-amino acids, the enzyme can also hydrolyze formyl and acetyl derivatives to varying degrees.

UniProtKB Entries (1)

Geobacillus stearothermophilus
N-carbamoyl-L-amino acid hydrolase

PDB Structure

External Links
Primary Citation
Mutational and structural analysis of L-N-carbamoylase reveals new insights into a peptidase m20/m25/m40 family member.
Martinez-Rodriguez, S., Garcia-Pino, A., Las Heras-Vazquez, F.J., Clemente-Jimenez, J.M., Rodriguez-Vico, F., Garcia-Ruiz, J.M., Loris, R., Gavira, J.A.