CATH Classification

Domain Context

CATH Clusters

Superfamily Cysteine proteinases
Functional Family Putative ubiquitin carboxyl-terminal hydrolase 8

Enzyme Information

3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt P40818
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (1)

P0CG48
UBC_HUMAN
Homo sapiens
Polyubiquitin-C

PDB Structure

PDB 3N3K
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
A strategy for modulation of enzymes in the ubiquitin system.
Ernst, A., Avvakumov, G., Tong, J., Fan, Y., Zhao, Y., Alberts, P., Persaud, A., Walker, J.R., Neculai, A.M., Neculai, D., Vorobyov, A., Garg, P., Beatty, L., Chan, P.K., Juang, Y.C., Landry, M.C., Yeh, C., Zeqiraj, E., Karamboulas, K., Allali-Hassani, A., Vedadi, M., Tyers, M., Moffat, J., Sicheri, F., Pelletier, L., Durocher, D., Raught, B., Rotin, D., Yang, J., Moran, M.F., Dhe-Paganon, S., Sidhu, S.S.
Science