CATH Domain 3mzsB00

CATH Classification

Level	CATH Code	Description
	1	Mainly Alpha
	1.10	Orthogonal Bundle
	1.10.630	Cytochrome p450
	1.10.630.10	Cytochrome P450

Domain Context

CATH Clusters

Superfamily	Cytochrome P450
Functional Family	Cholesterol side-chain cleavage enzyme, mitochondrial

Enzyme Information

1.14.15.6

Cholesterol monooxygenase (side-chain-cleaving).

based on mapping to UniProt P00189

Cholesterol + 6 reduced adrenodoxin + 3 O(2) + 6 H(+) = pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H(2)O.

-!- The reaction proceeds in three stages, with two hydroxylations at C-22 and C-20 preceding scission of the side-chain between carbons 20 and 22. -!- The initial source of the electrons is NADPH, which transfers the electrons to the adrenodoxin via EC 1.18.1.6.

UniProtKB Entries (1)

P00189

CP11A_BOVIN

Bos taurus

Cholesterol side-chain cleavage enzyme, mitochondrial

PDB Structure

PDB	3MZS
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structural Basis for Three-step Sequential Catalysis by the Cholesterol Side Chain Cleavage Enzyme CYP11A1. Mast, N., Annalora, A.J., Lodowski, D.T., Palczewski, K., Stout, C.D., Pikuleva, I.A. J.Biol.Chem.