CATH Classification

Domain Context

CATH Clusters

Superfamily Methyl-coenzyme M Reductase; Chain A, domain 1
Functional Family

Enzyme Information

2.8.4.1
Coenzyme-B sulfoethylthiotransferase.
based on mapping to UniProt P11558
Methyl-CoM + CoB = CoM-S-S-CoB + methane.
-!- This enzyme catalyzes the final step in methanogenesis, the biological production of methane. -!- This important anaerobic process is carried out only by methanogenic archaea. -!- The enzyme can also function in reverse, for anaerobic oxidation of methane. -!- The enzyme requires the hydroporphinoid nickel complex coenzyme F(430). -!- Highly specific for coenzyme B with a heptanoyl chain; ethyl CoM and difluoromethyl CoM are poor substrates. -!- The sulfide sulfur can be replaced by selenium but not by oxygen.

UniProtKB Entries (1)

P11558
MCRA_METTM
Methanothermobacter marburgensis str. Marburg
Methyl-coenzyme M reductase I subunit alpha

PDB Structure

PDB 3M32
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural insight into methyl-coenzyme M reductase chemistry using coenzyme B analogues.
Cedervall, P.E., Dey, M., Pearson, A.R., Ragsdale, S.W., Wilmot, C.M.
Biochemistry