CATH Classification

Domain Context

CATH Clusters

Superfamily Type I PLP-dependent aspartate aminotransferase-like (Major domain)
Functional Family Cysteine desulfurase IscS

Enzyme Information

2.8.1.7
Cysteine desulfurase.
based on mapping to UniProt P0A6B9
L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.
-!- The sulfur from free L-cysteine is first transferred to a cysteine residue in the active site, and then passed on to various other acceptors. -!- The enzyme is involved in the biosynthesis of iron-sulfur clusters, thio-nucleosides in tRNA, thiamine, biotin, lipoate and pyranopterin (molybdopterin). -!- In Azotobacter vinelandii, this sulfur provides the inorganic sulfide required for nitrogenous metallocluster formation.

UniProtKB Entries (1)

P0A6B9
ISCS_ECO57
Escherichia coli O157:H7
Cysteine desulfurase IscS

PDB Structure

PDB 3LVM
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural basis for Fe-S cluster assembly and tRNA thiolation mediated by IscS protein-protein interactions.
Shi, R., Proteau, A., Villarroya, M., Moukadiri, I., Zhang, L., Trempe, J.F., Matte, A., Armengod, M.E., Cygler, M.
Plos Biol.