CATH Classification

Domain Context

CATH Clusters

Superfamily Alpha-ketoglutarate-dependent dioxygenase AlkB-like
Functional Family Alpha-ketoglutarate-dependent dioxygenase AlkB

Enzyme Information

1.14.11.33
DNA oxidative demethylase.
based on mapping to UniProt P05050
DNA-base-CH(3) + 2-oxoglutarate + O(2) = DNA-base + formaldehyde + succinate + CO(2).
-!- Activity is slightly stimulated by ascorbate. -!- Catalyzes oxidative demethylation of the DNA base lesions N(1)- methyladenine, N(3)-methylcytosine, N(1)-methylguanine, and N(3)- methylthymine. -!- It works better on single-stranded DNA (ssDNA) and is capable of repairing damaged bases in RNA.

UniProtKB Entries (1)

P05050
ALKB_ECOLI
Escherichia coli K-12
Alpha-ketoglutarate-dependent dioxygenase AlkB

PDB Structure

PDB 3I3Q
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Enzymological and structural studies of the mechanism of promiscuous substrate recognition by the oxidative DNA repair enzyme AlkB.
Yu, B., Hunt, J.F.
Proc.Natl.Acad.Sci.USA