CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.970
Functional Family 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Enzyme Information

2.2.1.9
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase.
based on mapping to UniProt P17109
Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl- cyclohex-3-ene-1-carboxylate + CO(2).
-!- Involved in the biosynthesis of vitamin K(2) (menaquinone). -!- In most anaerobes and all Gram-positive aerobes, menaquinone is the sole electron transporter in the respiratory chain and is essential for their survival. -!- It had previously been thought that the products of the reaction were (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate (SHCHC), pyruvate and CO(2) but it is now known that two separate enzymes are involved: this enzyme and EC 4.2.99.20. -!- Under basic conditions, the product can spontaneously lose pyruvate to form SHCHC. -!- Formerly EC 2.5.1.64 and EC 2.5.1.n1.

UniProtKB Entries (1)

P17109
MEND_ECOLI
Escherichia coli K-12
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

PDB Structure

PDB 3FLM
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural insights of the MenD from Escherichia coli reveal ThDP affinity.
Priyadarshi, A., Saleem, Y., Nam, K.H., Kim, K.S., Park, S.Y., Kim, E.E., Hwang, K.Y.
Biochem.Biophys.Res.Commun.