CATH Domain 3fl3B00
|22.214.171.124||Ribonuclease A-like domain|
|Superfamily||Ribonuclease A-like domain|
|Functional Family||Ribonuclease pancreatic|
based on mapping to UniProt P61823
(1) An (RNA) containing cytidine + H(2)O = an (RNA)-3'-cytidine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA). (2) An (RNA) containing uridine + H(2)O = an (RNA)-3'-uridine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA).
-!- Specifically cleaves at the 3'-side of pyrimidine (uracil or cytosine) phosphate bonds in RNA. -!- The reaction takes place in two steps, with the 2',3'-cyclic phosphodiester intermediates released from the enzyme at the completion of the first step. -!- Hydrolysis of these cyclic compounds occurs at a much slower rate through a reversal of the first step, in which the -OH group of water substitutes for the 2'-OH group of the ribose used in the first step, and does not take place until essentially all the susceptible 3',5'-phosphodiester bonds have been cyclised. -!- The enzyme can act as an endo- or exo ribonuclease. -!- Formerly EC 126.96.36.199, EC 188.8.131.52 and EC 184.108.40.206.
UniProtKB Entries (1)
Toward an antitumor form of bovine pancreatic ribonuclease: The crystal structure of three noncovalent dimeric mutants