CATH Classification

Domain Context

CATH Clusters

Superfamily Cytochrome P450
Functional Family

Enzyme Information

1.14.14.46
Pimeloyl-[acyl-carrier protein] synthase.
based on mapping to UniProt P53554
A long-chain acyl-[acyl-carrier protein] + 2 reduced flavodoxin + 3 O(2) = pimeloyl-[acyl-carrier protein] + an n-alkanal + 2 oxidized flavodoxin + 3 H(2)O.
-!- Catalyzes an oxidative C-C bond cleavage of long-chain acyl-[acyl- carrier protein]s of various lengths to generate pimeloyl-[acyl- carrier protein], an intermediate in the biosynthesis of biotin. -!- The preferred substrate of the enzyme from the bacterium Bacillus subtilis is palmitoyl-[acyl-carrier protein] which then gives heptanal as the alkanal. -!- The mechanism is similar to EC 1.14.15.6, followed by a hydroxylation step, which may occur spontaneously. -!- Formerly EC 1.14.15.12.

UniProtKB Entries (2)

P0A6A8
ACP_ECOLI
Escherichia coli K-12
Acyl carrier protein
P53554
BIOI_BACSU
Bacillus subtilis subsp. subtilis str. 168
Biotin biosynthesis cytochrome P450

PDB Structure

PDB 3EJE
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural insights from a P450 Carrier Protein complex reveal how specificity is achieved in the P450(BioI) ACP complex.
Cryle, M.J., Schlichting, I.
Proc.Natl.Acad.Sci.Usa