CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.70.270
Functional Family Pol polyprotein

Enzyme Information

3.1.13.2
Exoribonuclease H.
based on mapping to UniProt P04585
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
-!- This is a secondary reaction to the RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end performed by EC 3.1.26.13.
3.1.-.-
Acting on ester bonds.
based on mapping to UniProt P04585
2.7.7.7
DNA-directed DNA polymerase.
based on mapping to UniProt P04585
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time. -!- Cannot initiate a chain de novo. -!- Requires a primer which may be DNA or RNA. -!- See also EC 2.7.7.49.
3.4.23.16
HIV-1 retropepsin.
based on mapping to UniProt P04585
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
-!- Present in human immunodeficiency virus type 1. -!- Contributes to the maturation of the viral particle, and is a target of antiviral drugs. -!- Active enzyme is a dimer of identical 11-kDa subunits. -!- Similar enzymes occur in other retroviruses. -!- Belongs to peptidase family A2.
2.7.7.-
Nucleotidyltransferases.
based on mapping to UniProt P04585
2.7.7.49
RNA-directed DNA polymerase.
based on mapping to UniProt P04585
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
-!- Catalyzes RNA-template-directed extension of the 3'-end of a DNA strand by one deoxynucleotide at a time. -!- Cannot initiate a chain de novo. -!- Requires a RNA or DNA primer. -!- DNA can also serve as template. -!- See also EC 2.7.7.7.
3.1.26.13
Retroviral ribonuclease H.
based on mapping to UniProt P04585
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
-!- Retroviral reverse transcriptase is a multifunctional enzyme responsible for viral replication. -!- To perform this task the enzyme combines two distinct activities. -!- The polymerase domain (EC 2.7.7.49) occupies the N-terminal two- thirds of the reverse transcriptase whereas the ribonuclease H domain comprises the C-terminal remaining one-third. -!- The RNase H domain of Moloney murine leukemia virus and Human immunodeficiency virus display two metal binding sites.

UniProtKB Entries (1)

P04585
POL_HV1H2
HIV-1 M:B_HXB2R
Gag-Pol polyprotein

PDB Structure

PDB 3DYA
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Design of annulated pyrazoles as inhibitors of HIV-1 reverse transcriptase
Sweeney, Z.K., Harris, S.F., Arora, S.F., Javanbakht, H., Li, Y., Fretland, J., Davidson, J.P., Billedeau, J.R., Gleason, S.K., Hirschfeld, D., Kennedy-Smith, J.J., Mirzadegan, T., Roetz, R., Smith, M., Sperry, S., Suh, J.M., Wu, J., Tsing, S., Villasenor, A.G., Paul, A., Su, G., Heilek, G., Hang, J.Q., Zhou, A.S., Jernelius, J.A., Zhang, F.J., Klumpp, K.
J.Med.Chem.