CATH Classification

Domain Context

CATH Clusters

Superfamily Ribonuclease A-like domain
Functional Family Ribonuclease pancreatic

Enzyme Information

4.6.1.18
Pancreatic ribonuclease.
based on mapping to UniProt P00669
(1) An (RNA) containing cytidine + H(2)O = an (RNA)-3'-cytidine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA). (2) An (RNA) containing uridine + H(2)O = an (RNA)-3'-uridine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA).
-!- Specifically cleaves at the 3'-side of pyrimidine (uracil or cytosine) phosphate bonds in RNA. -!- The reaction takes place in two steps, with the 2',3'-cyclic phosphodiester intermediates released from the enzyme at the completion of the first step. -!- Hydrolysis of these cyclic compounds occurs at a much slower rate through a reversal of the first step, in which the -OH group of water substitutes for the 2'-OH group of the ribose used in the first step, and does not take place until essentially all the susceptible 3',5'-phosphodiester bonds have been cyclised. -!- The enzyme can act as an endo- or exo ribonuclease. -!- Formerly EC 2.7.7.16, EC 3.1.4.22 and EC 3.1.27.5.

UniProtKB Entries (1)

P00669
RNS_BOVIN
Bos taurus
Seminal ribonuclease

PDB Structure

PDB 3DJV
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Mapping the ribonucleolytic active site of bovine seminal ribonuclease. The binding of pyrimidinyl phosphonucleotide inhibitors
Dossi, K., Tsirkone, V.G., Hayes, J.M., Matousek, J., Pouckova, P., Soucek, J., Zadinova, M., Zographos, S.E., Leonidas, D.D.
Eur.J.Med.Chem.