CATH Classification

Domain Context

CATH Clusters

Superfamily SirC, precorrin-2 dehydrogenase, C-terminal helical domain-like
Functional Family

Enzyme Information

1.3.1.76
Precorrin-2 dehydrogenase.
based on mapping to UniProt P61818
Precorrin-2 + NAD(+) = sirohydrochlorin + NADH.
-!- Catalyzes the second of three steps leading to the formation of siroheme from uroporphyrinogen III. -!- The first step involves the donation of two S-adenosyl-L-methionine- derived methyl groups to carbons 2 and 7 of uroporphyrinogen III to form precorrin-2 (EC 2.1.1.107) and the third step involves the chelation of ferrous iron to sirohydrochlorin to form siroheme (EC 4.99.1.4). -!- In Saccharomyces cerevisiae, the last two steps are carried out by a single bifunctional enzyme, Met8p. -!- In some bacteria, steps 1-3 are catalyzed by a single multifunctional protein called CysG, whereas in Bacillus megaterium, three separate enzymes carry out each of the steps, with SirC being responsible for the above reaction.

UniProtKB Entries (1)

P61818
SIRC_BACME
Bacillus megaterium
Precorrin-2 dehydrogenase

PDB Structure

PDB 3DFZ
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure and function of SirC from Bacillus megaterium: a metal-binding precorrin-2 dehydrogenase
Schubert, H.L., Rose, R.S., Leech, H.K., Brindley, A.A., Hill, C.P., Rigby, S.E.J., Warren, M.J.
Biochem.J.