CATH Classification

Domain Context

CATH Clusters

Superfamily Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
Functional Family

Enzyme Information
Glucosyl-3-phosphoglycerate synthase.
based on mapping to UniProt Q73WU1
NDP-glucose + 3-phospho-D-glycerate = NDP + 2-O-(alpha-D-glucopyranosyl)- 3-phospho-D-glycerate.
-!- The enzyme is involved in biosynthesis of 2-O-(alpha-D- glucopyranosyl)-D-glycerate via the two-step pathway in which glucosyl-3-phosphoglycerate synthase catalyzes the conversion of GDP- glucose and 3-phospho-D-glycerate into 2-O-(alpha-D-glucopyranosyl)- 3-phospho-D-glycerate, which is then converted to 2-O-(alpha-D- glucopyranosyl)-D-glycerate by EC -!- The activity is dependent on divalent cations (Mn(2+), Co(2+), or Mg(2+)). -!- The enzyme from Persephonella marina shows moderate flexibility on the sugar donor concerning the nucleotide moiety (UDP-glucose, ADP- glucose, GDP-glucose) but is strictly specific for glucose. -!- The enzyme is also strictly specific for 3-phospho-D-glycerate as acceptor. -!- The enzyme from Methanococcoides burtonii is strictly specific for GDP-glucose and 3-phospho-D-glycerate. -!- This enzyme catalyzes the first glucosylation step in methylglucose lipopolysaccharide biosynthesis in mycobacteria.

UniProtKB Entries (1)

Mycobacterium avium subsp. paratuberculosis K-10
Glucosyl-3-phosphoglycerate synthase

PDB Structure

External Links
Primary Citation
Crystal structure of a UDP-glucose-specific glycosyltransferase from a Mycobacterium species.
Fulton, Z., McAlister, A., Wilce, M.C., Brammananth, R., Zaker-Tabrizi, L., Perugini, M.A., Bottomley, S.P., Coppel, R.L., Crellin, P.K., Rossjohn, J., Beddoe, T.