CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.160.20
Functional Family endoribonuclease Dicer

Enzyme Information

3.1.26.3
Ribonuclease III.
based on mapping to UniProt Q8R418
Endonucleolytic cleavage to 5'-phosphomonoester.
-!- An endoribonuclease that cleaves double-stranded RNA molecules. -!- The cleavage can be either a single-stranded nick or double-stranded break in the RNA, depending in part upon the degree of base-pairing in the region of the cleavage site. -!- Specificity is conferred by negative determinants, i.e., the presence of certain Watson-Crick base-pairs at specific positions that strongly inhibit cleavage. -!- RNase III is involved in both rRNA processing and mRNA processing and decay.

UniProtKB Entries (1)

Q8R418
DICER_MOUSE
Mus musculus
Endoribonuclease Dicer

PDB Structure

PDB 3C4T
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural and biochemical insights into the dicing mechanism of mouse Dicer: A conserved lysine is critical for dsRNA cleavage.
Du, Z., Lee, J.K., Tjhen, R., Stroud, R.M., James, T.L.
Proc.Natl.Acad.Sci.Usa