CATH Classification

Domain Context

CATH Clusters

Superfamily Zn-finger domain of Sec23/24
Functional Family RanBP-type and C3HC4-type zinc finger-containing protein 1

Enzyme Information

2.3.2.31
RBR-type E3 ubiquitin transferase.
based on mapping to UniProt Q9WUB0
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.
-!- RBR-type E3 ubiquitin transferases have two RING fingers separated by an internal motif (IBR, for In Between RING). -!- The enzyme interacts with the CRL (Cullin-RING ubiquitin Ligase) complexes formed by certain RING-type E3 ubiquitin transferase (see EC 2.3.2.27), which include a neddylated cullin scaffold protein and a substrate recognition module. -!- The RING1 domain binds an EC 2.3.2.23, and transfers the ubiquitin that is bound to it to an internal cysteine residue in the RING2 domain, followed by the transfer of the ubiquitin from RING2 to the substrate. -!- Once the substrate has been ubiquitinated by the RBR-type ligase, it can be ubiqutylated further using ubiquitin carried directly on E2 enzymes, in a reaction catalyzed by EC 2.3.2.27. -!- Activity of the RBR-type enzyme is dependent on neddylation of the cullin protein in the CRL complex. -!- Cf. EC 2.3.2.26, EC 2.3.2.27, and EC 2.3.2.32.

UniProtKB Entries (2)

Q9WUB0
HOIL1_MOUSE
Mus musculus
RanBP-type and C3HC4-type zinc finger-containing protein 1
P0CG48
UBC_HUMAN
Homo sapiens
Polyubiquitin-C

PDB Structure

PDB 3B08
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Specific recognition of linear ubiquitin chains by the Npl4 zinc finger (NZF) domain of the HOIL-1L subunit of the linear ubiquitin chain assembly complex
Sato, Y., Fujita, H., Yoshikawa, A., Yamashita, M., Yamagata, A., Kaiser, S.E., Iwai, K., Fukai, S.
Proc.Natl.Acad.Sci.USA