CATH Classification

Domain Context

CATH Clusters

Superfamily N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain
Functional Family tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase

Enzyme Information

2.1.1.215
tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase.
based on mapping to UniProt O67010
4 S-adenosyl-L-methionine + guanine(26)/guanine(27) in tRNA = 4 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26)/N(2)- dimethylguanine(27) in tRNA.
-!- The enzyme from Aquifex aeolicus is similar to the TRM1 methyltransferases of archaea and eukarya (see EC 2.1.1.216). -!- However, it catalyzes the double methylation of guanines at both positions 26 and 27 of tRNA. -!- Formerly EC 2.1.1.32.

UniProtKB Entries (1)

O67010
TRM1_AQUAE
Aquifex aeolicus VF5
TRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase

PDB Structure

PDB 3AXT
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Substrate tRNA recognition mechanism of a multisite-specific tRNA methyltransferase, Aquifex aeolicus Trm1, based on the X-ray crystal structure
Awai, T., Ochi, A., Ihsanawati, Sengoku, T., Hirata, A., Bessho, Y., Yokoyama, S., Hori, H.
J.Biol.Chem.