CATH Classification

Domain Context

CATH Clusters

Superfamily Oligo-1,6-glucosidase; Domain 2
Functional Family Oligo-1,6-glucosidase

Enzyme Information

3.2.1.10
Oligo-1,6-glucosidase.
based on mapping to UniProt P53051
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.
-!- This enzyme, like EC 3.2.1.33, can release an alpha-1->6-linked glucose, whereas the shortest chain that can be released by EC 3.2.1.41, EC 3.2.1.142 and EC 3.2.1.68 is maltose. -!- It also hydrolyzes isomaltulose (palatinose), isomaltotriose and panose, but has no action on glycogen or phosphorylase limit dextrin. -!- The enzyme from intestinal mucosa is a single polypeptide chain that also catalyzes the reaction of EC 3.2.1.48. -!- Differs from EC 3.2.1.33 in its preference for short-chain substrates and in its not requiring the 6-glucosylated residue to be at a branch point, i.e. linked at both C-1 and C-4.

UniProtKB Entries (1)

P53051
MALX3_YEAST
Saccharomyces cerevisiae S288C
Oligo-1,6-glucosidase IMA1

PDB Structure

PDB 3AXI
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Steric hindrance by 2 amino acid residues determines the substrate specificity of isomaltase from Saccharomyces cerevisiae
Yamamoto, K., Miyake, H., Kusunoki, M., Osaki, S.
J.Biosci.Bioeng.