CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.10 | Roll |
|
3.10.450 | Nuclear Transport Factor 2; Chain: A, |
|
3.10.450.30 | Microbial ribonucleases |
Domain Context
CATH Clusters
| Superfamily | Microbial ribonucleases |
| Functional Family |
Enzyme Information
| 4.6.1.20 |
Ribonuclease U(2).
based on mapping to UniProt P00654
(1) (RNA) containing adenosine + H(2)O = an (RNA fragment)-3'-adenosine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment). (2) (RNA) containing guanosine + H(2)O = an (RNA fragment)-3'-guanosine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment).
-!- The enzyme secreted by the fungus Ustilago sphaerogena cleaves at the 3'-phosphate group of purines, and catalyzes a two-stage endonucleolytic cleavage. -!- The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending in Ap or Gp with 2',3'-cyclic phosphodiester, which are released from the enzyme. -!- The enzyme then hydrolyzes these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. -!- The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. -!- The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides. -!- Formerly EC 3.1.27.4.
|
UniProtKB Entries (1)
| P00654 |
RNU2_USTSP
Ustilago sphaerogena
Ribonuclease U2
|
PDB Structure
| PDB | 3AGO |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate
Acta Crystallogr.,Sect.D
|