CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
1 | Mainly Alpha |
|
1.10 | Orthogonal Bundle |
|
1.10.540 | Butyryl-Coa Dehydrogenase, subunit A; domain 1 |
|
1.10.540.10 | Acyl-CoA dehydrogenase/oxidase, N-terminal domain |
Domain Context
CATH Clusters
| Superfamily | Acyl-CoA dehydrogenase/oxidase, N-terminal domain |
| Functional Family | Flavin-dependent monooxygenase, oxygenase subunit HsaA |
Enzyme Information
| 1.14.14.12 |
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase.
based on mapping to UniProt P9WJA1
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH(2) + O(2) = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H(2)O.
-!- This bacterial enzyme participates in the degradation of several steroids, including cholesterol and testosterone. -!- It can use either FADH or FMNH(2) as flavin cofactor. -!- The enzyme forms a two-component system with a reductase (HsaB) that utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit.
|
UniProtKB Entries (1)
| P9WJA1 |
HSAA_MYCTU
Mycobacterium tuberculosis H37Rv
Flavin-dependent monooxygenase, oxygenase subunit HsaA
|
PDB Structure
| PDB | 3AFF |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism
J.Biol.Chem.
|