CATH Classification

Domain Context

CATH Clusters

Superfamily NAD(P)-binding Rossmann-like Domain
Functional Family Acetoin dehydrogenase (Diacetyl reductase)

Enzyme Information
(S,S)-butanediol dehydrogenase.
based on mapping to UniProt Q9ZNN8
(2S,3S)-butane-2,3-diol + NAD(+) = (S)-acetoin + NADH.
-!- This enzyme catalyzes the reversible reduction of (S)-acetoin to (S,S)-butane-2,3-diol. -!- It can also catalyze the irreversible reduction of diacetyl to (S)- acetoin.
Diacetyl reductase ((S)-acetoin forming).
based on mapping to UniProt Q9ZNN8
(S)-acetoin + NAD(+) = diacetyl + NADH.
-!- The reaction is catalyzed in the reverse direction. -!- This activity is usually associated with butanediol dehydrogenase activity (EC or EC -!- While the butanediol dehydrogenase activity is reversible, diacetyl reductase activity is irreversible. -!- This enzyme has been reported in the bacteria Geobacillus stearothermophilus, Enterobacter aerogenes and Klebsiella pneumoniae. -!- Different from EC -!- Formerly EC

UniProtKB Entries (1)

Corynebacterium glutamicum
L-2,3-butanediol dehydrogenase

PDB Structure

PDB 3A28
External Links
Primary Citation
Structural basis for chiral substrate recognition by two 2,3-butanediol dehydrogenases
Otagiri, M., Ui, S., Takusagawa, Y., Ohtsuki, T., Kurisu, G., Kusunoki, M.
Febs Lett.