CATH Classification

Domain Context

CATH Clusters

Superfamily tRNA(Met) cytidine acetyltransferase, tail domain
Functional Family tRNA(Met) cytidine acetyltransferase TmcA

Enzyme Information

2.3.1.193
tRNA(Met) cytidine acetyltransferase.
based on mapping to UniProt P76562
(Elongator tRNA(Met))-cytidine(34) + ATP + acetyl-CoA + H(2)O = (elongator tRNA(Met))-N(4)-acetylcytidine(34) + ADP + phosphate + CoA.
-!- The enzyme acetylates the wobble base C34 of the CAU anticodon of elongation-specific tRNA(Met). -!- Escherichia coli TmcA strictly discriminates elongator tRNA(Met) from tRNA(Ile), which is structurally similar and has the same anticodon loop, mainly by recognizing the C27-G43 pair in the anticodon stem. -!- The enzyme can use GTP in place of ATP for formation of N(4)- acetylcytidine.

UniProtKB Entries (1)

P76562
TMCA_ECOLI
Escherichia coli K-12
TRNA(Met) cytidine acetyltransferase TmcA

PDB Structure

PDB 2ZPA
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
RNA helicase module in an acetyltransferase that modifies a specific tRNA anticodon
Chimnaronk, S., Suzuki, T., Manita, T., Ikeuchi, Y., Yao, M., Suzuki, T., Tanaka, I.
Embo J.