CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
1 | Mainly Alpha |
|
1.10 | Orthogonal Bundle |
|
1.10.287 | Helix Hairpins |
|
1.10.287.540 | Helix hairpin bin |
Domain Context
CATH Clusters
| Superfamily | Helix hairpin bin |
| Functional Family |
Enzyme Information
| 6.1.1.26 |
Pyrrolysine--tRNA(Pyl) ligase.
based on mapping to UniProt Q8PWY1
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl- tRNA(Pyl).
-!- In organisms such as Methanosarcina barkeri that incorporate the modified amino acid pyrrolysine (Pyl) into certain methylamine methyltransferases, an unusual tRNA(Pyl), with a CUA anticodon, can be charged directly with pyrrolysine by this class II aminoacyl- -tRNA ligase. -!- Specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids.
|
UniProtKB Entries (1)
| Q8PWY1 |
PYLS_METMA
Methanosarcina mazei Go1
Pyrrolysine--tRNA ligase
|
PDB Structure
| PDB | 2ZIO |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode N(varepsilon)-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification
Chem.Biol.
|