CATH Classification

Domain Context

CATH Clusters

Superfamily 2.40.128.390
Functional Family

Enzyme Information

2.4.99.21
Dolichyl-phosphooligosaccharide-protein glycotransferase.
based on mapping to UniProt Q8U4D2
An archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
-!- The enzyme, characterized from the archaea Methanococcus voltae and Haloferax volcanii, transfers a glycan component from dolichyl phosphooligosaccharide to external proteins. -!- It is different from EC 2.4.99.18 which uses dolichyl diphosphate as carrier compound in bacteria and eukaryotes. -!- The enzyme participates in the N-glycosylation of proteins in some archaea. -!- Dolichol used by archaea is different from that used by eukaryotes; it is much shorter (C(55)-C(60)), it is alpha,omega-saturated and it may have additional unsaturated positions in the chain.

UniProtKB Entries (1)

Q8U4D2
AGLB1_PYRFU
Pyrococcus furiosus DSM 3638
Dolichyl-phosphooligosaccharide-protein glycotransferase 1

PDB Structure

PDB 2ZAG
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure-guided identification of a new catalytic motif of oligosaccharyltransferase
Igura, M., Maita, N., Kamishikiryo, J., Yamada, M., Obita, T., Maenaka, K., Kohda, D.
Embo J.