CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.360
Functional Family FMN-dependent NADH-azoreductase

Enzyme Information

1.7.1.17
FMN-dependent NADH-azoreductase.
based on mapping to UniProt P41407
Anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH.
-!- The enzyme catalyzes the reductive cleavage of an azo bond in aromatic azo compounds to form the corresponding amines. -!- Does not accept NADPH. -!- Cf. EC 1.7.1.6.

UniProtKB Entries (1)

P41407
AZOR_ECOLI
Escherichia coli K-12
FMN-dependent NADH-azoreductase

PDB Structure

PDB 2Z9C
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Expansion of Substrate Specificity and Catalytic Mechanism of Azoreductase by X-ray Crystallography and Site-directed Mutagenesis
Ito, K., Nakanishi, M., Lee, W.C., Zhi, Y., Sasaki, H., Zenno, S., Saigo, K., Kitade, Y., Tanokura, M.
J.Biol.Chem.