CATH Classification

Domain Context

CATH Clusters

Superfamily 6.20.240.50
Functional Family

Enzyme Information

3.4.22.-
Cysteine endopeptidases.
based on mapping to UniProt Q01499
2.7.7.48
RNA-directed RNA polymerase.
based on mapping to UniProt Q01499
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes RNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- See also EC 2.7.7.6.
3.4.21.113
Pestivirus NS3 polyprotein peptidase.
based on mapping to UniProt Q01499
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites.
-!- The polyprotein of noncytopathogenic pestiviruses is cleaved co- and post-translationally into at least 11 proteins (N(pro), C, E(rns), E1, E2, p7, NS2-3, NS4A, NS4B, NS5A, and NS5B). -!- The genomes of cytopathogenic pestivirus strains express at least one additional protein, called NS3 (p80). -!- This enzyme, which resides in the N-terminal region of NS3 (nonstructural protein 3), is essential for generation of its own C-terminus and for processing of the downstream cleavage sites, leading to the release of the pestivirus nonstructural proteins NS4A, NS4B, NS5A and NS5B. -!- Belongs to peptidase family S31.
3.6.4.13
RNA helicase.
based on mapping to UniProt Q01499
ATP + H(2)O = ADP + phosphate.
-!- RNA helicases utilize the energy from ATP hydrolysis to unwind RNA. -!- Some of them unwind RNA with a 3' to 5' polarity, other show 5' to 3' polarity. -!- Some helicases unwind DNA as well as RNA. -!- May be identical with EC 3.6.4.12 (DNA helicase).
3.6.1.15
Nucleoside-triphosphate phosphatase.
based on mapping to UniProt Q01499
NTP + H(2)O = NDP + phosphate.
-!- The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. -!- Also hydrolyzes nucleoside diphosphates, thiamine diphosphate and FAD. -!- The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin.

UniProtKB Entries (1)

Q01499
POLG_BVDVS
Bovine viral diarrhea virus 1-SD1
Genome polyprotein

PDB Structure

PDB 2YQ3
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure of a Pestivirus Envelope Glycoprotein E2 Clarifies its Role in Cell Entry.
El Omari, K., Iourin, O., Harlos, K., Grimes, J.M., Stuart, D.I.
Cell Rep.