CATH Classification

Domain Context

CATH Clusters

Superfamily 3.10.450.50
Functional Family Biphenyl dioxygenase subunit beta

Enzyme Information

1.14.12.18
Biphenyl 2,3-dioxygenase.
based on mapping to UniProt P37334
Biphenyl + NADH + O(2) = (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD(+).
-!- The enzyme from Burkholderia fungorum strain LB400 (previously Pseudomonas sp.) is part of a multicomponent system composed of an NADH:ferredoxin oxidoreductase (FAD cofactor), a [2Fe-2S] Rieske-type ferredoxin, and a terminal oxygenase that contains a [2Fe-2S] Rieske- type iron-sulfur cluster and a catalytic mononuclear nonheme iron center. -!- Chlorine-substituted biphenyls can also act as substrates. -!- Similar to the three-component enzyme systems EC 1.14.12.3 and EC 1.14.12.11.

UniProtKB Entries (1)

P37334
BPHE_PARXL
Paraburkholderia xenovorans LB400
Biphenyl dioxygenase subunit beta

PDB Structure

PDB 2YFI
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Retuning Rieske-Type Oxygenases to Expand Substrate Range.
Mohammadi, M., Viger, J., Kumar, P., Barriault, D., Bolin, J.T., Sylvestre, M.
J.Biol.Chem.