CATH Classification

Domain Context

CATH Clusters

Superfamily Ubiquitin Conjugating Enzyme
Functional Family Ubiquitin-conjugating enzyme E2 B

Enzyme Information

2.3.2.23
E2 ubiquitin-conjugating enzyme.
based on mapping to UniProt P63146
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.
-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.

UniProtKB Entries (1)

P63146
UBE2B_HUMAN
Homo sapiens
Ubiquitin-conjugating enzyme E2 B

PDB Structure

PDB 2YB6
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
E3 Ligase Rad18 Promotes Monoubiquitination Rather Than Ubiquitin Chain Formation by E2 Enzyme Rad6.
Hibbert, R.G., Huang, A., Boelens, R., Sixma, T.K.
Proc.Natl.Acad.Sci.USA