CATH Classification

Domain Context

CATH Clusters

Superfamily Clavaminate synthase-like
Functional Family Hypoxia-inducible factor 1-alpha inhibitor

Enzyme Information

1.14.11.n4
Ankyrin-repeat-histidine dioxagenase.
based on mapping to UniProt Q9NWT6
Ankyrin-repeat-L-histidine + 2-oxoglutarate + O(2) = ankyrin-repeat-(3S)- 3-hydroxy-L-histidine + succinate + CO(2).
1.14.11.30
Hypoxia-inducible factor-asparagine dioxygenase.
based on mapping to UniProt Q9NWT6
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia- inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2).
-!- Contains iron, and requires ascorbate. -!- Catalyzes hydroxylation of an asparagine in the C-terminal transcriptional activation domain of HIF-alpha, the alpha subunit of the transcriptional regulator HIF (hypoxia-inducible factor), which reduces its interaction with the transcriptional coactivator protein p300. -!- The requirement of oxygen for the hydroxylation reaction enables animals to respond to hypoxia.

UniProtKB Entries (1)

Q9NWT6
HIF1N_HUMAN
Homo sapiens
Hypoxia-inducible factor 1-alpha inhibitor

PDB Structure

PDB 2Y0I
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains.
Yang, M., Chowdhury, R., Ge, W., Hamed, R.B., McDonough, M.A., Claridge, T.D., Kessler, B.M., Cockman, M.E., Ratcliffe, P.J., Schofield, C.J.
FEBS J.