CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.1400
Functional Family Sirohydrochlorin cobaltochelatase

Enzyme Information

4.99.1.3
Sirohydrochlorin cobaltochelatase.
based on mapping to UniProt Q05592
Cobalt-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Co(2+).
-!- This enzyme is a type II chelatase, being either a monomer (CbiX) or a homodimer (CibK) and being ATP-independent. -!- CbiK from Salmonella enterica uses precorrin-2 as the substrate to yield cobalt-precorrin-2. -!- The enzyme contains two histidines at the active site that are thought to be involved in the deprotonation of the tetrapyrrole substrate as well as in metal binding. -!- CbiX from Bacillus megaterium inserts cobalt at the level of sirohydrochlorin (factor-II) rather than precorrin-2.

UniProtKB Entries (1)

Q05592
CBIK_SALTY
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Sirohydrochlorin cobaltochelatase

PDB Structure

PDB 2XWP
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Evolution in a Family of Chelatases Facilitated by the Introduction of Active Site Asymmetry and Protein Oligomerization.
Romao, C.V., Ladakis, D., Lobo, S.A., Carrondo, M.A., Brindley, A.A., Deery, E., Matias, P.M., Pickersgill, R.W., Saraiva, L.M., Warren, M.J.
Proc.Natl.Acad.Sci.USA