CATH Domain 2xrxE01

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.90	Alpha-Beta Complex
	3.90.380	Naphthalene 1,2-dioxygenase Alpha Subunit; Chain A, domain 1
	3.90.380.10	Naphthalene 1,2-dioxygenase Alpha Subunit; Chain A, domain 1

Domain Context

CATH Clusters

Superfamily	Naphthalene 1,2-dioxygenase Alpha Subunit; Chain A, domain 1
Functional Family

Enzyme Information

1.14.12.18

Biphenyl 2,3-dioxygenase.

based on mapping to UniProt P37333

Biphenyl + NADH + O(2) = (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD(+).

-!- The enzyme from Burkholderia fungorum strain LB400 (previously Pseudomonas sp.) is part of a multicomponent system composed of an NADH:ferredoxin oxidoreductase (FAD cofactor), a [2Fe-2S] Rieske-type ferredoxin, and a terminal oxygenase that contains a [2Fe-2S] Rieske- type iron-sulfur cluster and a catalytic mononuclear nonheme iron center. -!- Chlorine-substituted biphenyls can also act as substrates. -!- Similar to the three-component enzyme systems EC 1.14.12.3 and EC 1.14.12.11.

UniProtKB Entries (1)

P37333

BPHA_PARXL

Paraburkholderia xenovorans LB400

Biphenyl dioxygenase subunit alpha

PDB Structure

PDB	2XRX
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structural Insight Into the Expanded Pcb-Degrading Abilities of a Biphenyl Dioxygenase Obtained by Directed Evolution. Kumar, P., Mohammadi, M., Viger, J.F., Barriault, D., Gomez-Gil, L., Eltis, L.D., Bolin, J.T., Sylvestre, M. J.Mol.Biol.