CATH Classification

Domain Context

CATH Clusters

Superfamily Cupin
Functional Family

Enzyme Information

1.14.11.27
[Histone H3]-dimetyl-L-lysine-36 demethylase.
based on mapping to UniProt Q9UPP1
[Protein]-N(6),N(6)-dimethyl-L-lysine + 2 2-oxoglutarate + 2 O(2) = [protein]-L-lysine + 2 succinate + 2 formaldehyde + 2 CO(2).
-!- Of the seven potential methylation sites in histones H3 (K4, K9, K27, K36, K79) and H4 (K20, R3) from HeLa cells, the enzyme is specific for Lys-36. -!- Lysine residues exist in three methylation states (mono-, di- and trimethylated). -!- The enzyme preferentially demethylates the dimethyl form of Lys-36 (K36me2), which is its natural substrate, to form the monomethyl and unmethylated forms of Lys-36. -!- It can also demethylate the monomethyl- but not the trimethyl form of Lys-36.

UniProtKB Entries (1)

Q9UPP1
PHF8_HUMAN
Homo sapiens
Histone lysine demethylase PHF8

PDB Structure

PDB 2WWU
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal structure of the PHF8 Jumonji domain, an Nepsilon-methyl lysine demethylase.
Yue, W.W., Hozjan, V., Ge, W., Loenarz, C., Cooper, C.D., Schofield, C.J., Kavanagh, K.L., Oppermann, U., McDonough, M.A.
FEBS Lett.