CATH Classification

Domain Context

CATH Clusters

Superfamily Clavaminate synthase-like
Functional Family Hypoxia-inducible factor 1-alpha inhibitor

Enzyme Information

1.14.11.n4
Ankyrin-repeat-histidine dioxagenase.
based on mapping to UniProt Q9NWT6
(1) [Ankyrin-repeat domain protein]-L-histidine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-histidine + succinate + CO(2). (2) [Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2). (3) [Ankyrin-repeat domain protein]-L-aspartate + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-aspartate + succinate + CO(2).
1.14.11.30
Hypoxia-inducible factor-asparagine dioxygenase.
based on mapping to UniProt Q9NWT6
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia- inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2).
-!- Contains iron, and requires ascorbate. -!- Catalyzes hydroxylation of an asparagine in the C-terminal transcriptional activation domain of HIF-alpha, the alpha subunit of the transcriptional regulator HIF (hypoxia-inducible factor), which reduces its interaction with the transcriptional coactivator protein p300. -!- The requirement of oxygen for the hydroxylation reaction enables animals to respond to hypoxia.

UniProtKB Entries (1)

Q9NWT6
HIF1N_HUMAN
Homo sapiens
Hypoxia-inducible factor 1-alpha inhibitor

PDB Structure

PDB 2W0X
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural basis for binding of cyclic 2-oxoglutarate analogues to factor-inhibiting hypoxia-inducible factor.
Conejo-Garcia, A., McDonough, M.A., Loenarz, C., McNeill, L.A., Hewitson, K.S., Ge, W., Lienard, B.M., Schofield, C.J., Clifton, I.J.
Bioorg. Med. Chem. Lett.