CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.47.10
Functional Family Acetyl-CoA acetyltransferase

Enzyme Information

2.3.1.9
Acetyl-CoA C-acetyltransferase.
based on mapping to UniProt P07097
2 acetyl-CoA = CoA + acetoacetyl-CoA.
-!- The enzyme, found in both eukaryotes and prokaryotes, catalyzes the Claisen condensation of an acetyl-CoA and an acyl-CoA (often another acetyl-CoA), leading to the formation of an acyl-CoA that is longer by two carbon atoms. -!- The reaction starts with the acylation of a nucleophilic cysteine at the active site, usually by acetyl-CoA but potentially by a different acyl-CoA, with concomitant release of CoA. -!- In the second step the acyl group is transferred to an acetyl-CoA molecule. -!- Cf. EC 2.3.1.16.

UniProtKB Entries (1)

P07097
THIL_ZOORA
Zoogloea ramigera
Acetyl-CoA acetyltransferase

PDB Structure

PDB 2VU0
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme.
Merilainen, G., Schmitz, W., Wierenga, R.K., Kursula, P.
FEBS J.