CATH Classification

Domain Context

CATH Clusters

Superfamily 2.60.120.200
Functional Family

Enzyme Information

2.4.2.36
NAD(+)--diphthamide ADP-ribosyltransferase.
based on mapping to UniProt Q5EK40
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2].
-!- Dihtheria toxin and some other bacterial toxins catalyze this reaction, which inactivates translation elongation factor 2 (EF2). -!- The acceptor is diphthamide, a unique modification of a histidine residue in the elongation factor found in archaebacteria and all eukaryotes, but not in eubacteria. -!- Cf. EC 2.4.2.31. -!- The relevant histidine of EF2 is His(715) in mammals, His(699) in yeast and His(600) in Pyrococcus horikoshii.

UniProtKB Entries (1)

Q5EK40
CHXA_VIBCL
Vibrio cholerae
Cholix toxin

PDB Structure

PDB 2Q5T
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Cholix Toxin, a Novel ADP-ribosylating Factor from Vibrio cholerae.
Jorgensen, R., Purdy, A.E., Fieldhouse, R.J., Kimber, M.S., Bartlett, D.H., Merrill, A.R.
J.Biol.Chem.