CATH Classification

Domain Context

CATH Clusters

Superfamily PCRA; domain 4
Functional Family ATP-dependent DNA helicase

Enzyme Information

3.6.4.12
DNA helicase.
based on mapping to UniProt P56255
ATP + H(2)O = ADP + phosphate.
-!- DNA helicases utilize the energy from ATP hydrolysis to unwind double-stranded DNA. -!- Some of them unwind duplex DNA with a 3' to 5' polarity (1,3,5,8), other show 5' to 3' polarity (10,11,12,13) or unwind DNA in both directions (14,15). -!- Some helicases unwind DNA as well as RNA (4,9). -!- May be identical with EC 3.6.4.13 (RNA helicase).

UniProtKB Entries (1)

P56255
PCRA_GEOSE
Geobacillus stearothermophilus
ATP-dependent DNA helicase PcrA

PDB Structure

PDB 2PJR
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism.
Velankar, S.S., Soultanas, P., Dillingham, M.S., Subramanya, H.S., Wigley, D.B.
Cell(Cambridge,Mass.)